Figure 2. PHA-4 is expressed in the developing digestive tract. (A-F) PHA-4 (red) and MH27 adherens junction (green) staining. (A) pha-4
) null embryos. (B) 4E stage embryo (expression in ABaraaaa/p, ABaraapa/p, ABarapaa/p, ABalpaaa/p, ABalpapa/p, all MS great-granddaughters, midgut). (C) 8E stage embryo (expression in MSaaaa/p, MSaapa, MSpaaa/p, MSpapa, ABa descendants, midgut). (D-E) Midstage embryos [97+/-3 PHA-4+ cells in the head (expression in all pharyngeal cells, pharynx-intestinal valve cells, and arcade cells, based on cell position and double staining (n=3)], rectal cells (vir, rep, KK'), and midgut. (F) Terminal embryo (expression in entire pharynx, midgut, and rectum). (Arrow) Pharynx; (arrowhead) midgut; (*) rectum. Nuclear positions in pha-4
(+) (G) and pha-4
(-) (H) embryos at the 8E stage, ventral view. Pharyngeal cells are colored according to their lineal ancestry (yellow, ABalp; pink, ABara; green, MS). Midgut cells are red and rectal cells are blue. The embryos in B andC are rotated relative to the embryos shown here. Anterior is left (A-H); each embryo is ~50 μm.