Figure S4, related to Figure 2. JKK-1 and JNK-1 Function Cell Autonomously in Neurons and Are Enriched at the Presynaptic Terminals (A) Percentage rescue of
jkk-1 suppression of
arl-8 mutant phenotype in DA9 by wild-type
jkk-1 expressed using the Pitr-1 pB, Pmig-13, or heatshock (Phsp) promoter and percentage rescue of
jnk-1 suppression of
arl-8 mutant phenotype in DA9 by wild-type
jnk-1 expressed using the Pitr-1 pB or Pmig-13 promoter, or a kinase dead JNK-1 mutant expressed using the Pmig-13 promoter (wyEx4824, wyEx4875, wyEx4983, wyEx4860, wyEx4883, and wyEx5275, respectively). n=95-113 animals. (B-G) Colocalization of JKK-1::YFP (B, E) with mCherry::RAB-3 (C, F), double label (D, G), high magnification (E-G) in DA9 (wyEx4825). (H-J) Enrichment of JKK-1::YFP (H) over cytosolic mCherry (I), quantified by line scan of fluorescence intensity along the DA9 dorsal axon (J) (wyEx4732). (K-P) Colocalization of JNK-1::YFP (K, N) with mCherry::RAB-3 (L, O), double label (M, P), high magnification (N-P) in DA9 (wyEx4856). (Q-S) Enrichment of JNK-1::YFP (Q) over cytosolic mCherry (R), quantified by line scan of fluorescence intensity along the DA9 dorsal axon (S) (wyEx4756). Scale bars, 10 um. Note: ventral cord staining is from the motor neuron VA12 (bracket), labelled by the Pmig-13 promoter.