Figure 5. PAR-6 distribution in early embryos. Micrographs of embryos, wild type (A-F), par-6
) (G), par-3
) (H), co-stained with anti-PAR-6 and DAPI. The pkc-3
(RNAi) embryos were co-stained with anti-PAR-6 (I), anti-PAR-3 (J), and DAPI. (A) 1-cell embryo in meiosis II. (B) 1-cell embryo at anaphase of the first mitosis. (C) 2-cell embryo, when P1 is in prophase. Arrows indicate the posterior extent of PAR-6 signal in P1. (D) Late 2-cell embryo,when P1 is in anaphase. Arrowhead indicates posterior extent of PAR-6 signal on the ventral side. (E) 4-cell embryo. Note the weak signal at the periphery of prophase stage P2 cell. (F) Seven cell embryo, when P2 is in metaphase. Arrowhead indicates the ventral boundary of PAR-6 staining. (G) Late 1-cell stage par-6
) embryo. (H) Late 1-cell stage par-3
) embryo. (I) Anti-PAR-6 staining in an early 1-cell pkc-3
(RNAi) embryo. (J) Anti-PAR-3 staining in the same pkc-3
(RNAi) embryo shown in I. Note the weak and patchy signal of PAR-3 on the periphery. The high levels of cytoplasmic staining in this embryo are due to a higher background staining with this antibody. Scale bar, 9 µm.