Figure 5. PAL-1 distribution in
par-2,
par-3 and
par-4 mutant embryos. Fluorescence micrographs showing fixed 4-cell-stage wild-type and par mutant embryos stained with antibodies specific for PAL-1 (right column) and with DAPI (left column). See Table 3 for quantitation. (A,G) Wild-type embryo with high levels of PAL-1 in P2and EMS.(B,H)
par-2(
lw32) 4-cell-stage embryos usually have PAL-1 present only in two posterior blastomeres (H); one anterior nucleus is out of the focal plane (B). (C-E, I-K)
par-3(
it71) mutant embryos. PAL-1 is present only in the posterior blastomeres of some 4-cell-stage embryos (I), at higher levels posteriorly than anteriorly (J), and sometimes at equal levels in all blastomeres, as in an 8-cell-stage embryo (K). PAL-1 is not detectable in many
par-3 mutant embryos (see Table 3). (F,L)
par-4(
it57ts) 4-cell-stage mutant embryo with no detectable PAL-1 (L).