Figure 2. Expression of
clec-67 is controlled by ELT-2. (A) Pclec-67::gfp nematodes were grown on E. coli OP50 and were visualized using a Leica MZ FLIII fluorescence stereomicroscope. (B) Microarray (white) and qRT-PCR (light grey) comparing expression of
clec-67 in wild-type nematodes grown on S. enterica SL1344 versus wild-type nematodes grown on E. coli OP50. qRT-PCR comparing expression of
clec-67 in wild-type nematodes grown on E. coli carrying a vector control versus wild-type nematodes grown on E. coli expressing
elt-2 double-stranded RNA (dark grey). Results are the average of 3-7 independent experiments. Error bars represent SEM. (C) qRT-PCR comparing expression of a variety of intestinal housekeeping genes in wild-type nematodes grown on E. coli carrying a vector control versus wild-type nematodes grown on E. coli expressing
elt-2 double-stranded RNA. Results are the average of 5 independent experiments. Error bars represent SEM.