Fig. 1. Pcdc-25.2::gfp transgene was expressed in multiple somatic tissues in males. (A) A transcription unit of
cdc-25.2 (F16B4.8, isoform a). The region used as a promoter of
cdc25.2 in this study is indicated with a bracket. Respective symbols represent the following gene structures: black rectangles, exons; bridging gaps, introns; white rectangles, untranslated regions; a gray rectangle, an upstream region (2.9 kb). Scale bar, 1 kb. (BeK) Live images of Pcdc-25.2::gfp somatic expression with corresponding DIC images in males.Arrows indicate Pcdc-25.2::gfp signals, which were detected in seam cells (B, C), pharyngeal muscles (PM) and neurons (D, E), ventral nerve cords (VNC) (F, G), body muscles (BM) and diagonal muscles (DM) (H, I), and hypodermis (hyp) in male tails (J, K). The Pcdc-25.2::gfp signals were observed at the L3 larval (B, C), adult (DeI), and the L4 larval (J, K) stagemales, respectively. L3 and L4 larval stage males were distinguished with retraction of tail tip. Scale bars, 20 um. Insets show magnified images of a seam cell (C, scale bar, 10 um),diagonal muscles (I, scale bar, 5 um), and hypodermis (K, scale bar, 2 um), respectively