(G-L) Lateral view of a staged 3-3.5 hr posthatching L1 with full length
nfm-1::gfp and Pegl-17::mCherry expression. (G) Fluorescent micrograph ofGFP expression from
nfm-1::gfp rescuing fosmid. Asterisk marks URX expression of Pgcy-32::yfp in the head that was not excluded by GFP filter. The dashed rectangle indicates the enlarged posterior section in J-L. (H) Pegl-17::mCherry, fluorescent micrograph showing location of early Q neuroblasts. QL is out of focus because QL and QR are on different planes, QR on the right side and QL on left side of the animal. (I) Merge of A and B. No overlap of mCherry and GFP was observed. (J-L) Enlarged posterior section of G-I. (J) Enlargement of A to show
nfm-1::gfp present in posterior region near the anus. (K) Enlargement of B. QL is outlined to distinguish it from the V5 seam cell that transiently expresses Pegl-17. (L) Enlargement of C. Bar, 10 um. In all micrographs, anterior is to the left.