Figure 2. NCC-1 protein localization in wild-type and
ncc-1(RNAi) germ cells and embryos. (A-G) Staining of NCC-1 is in green and DNA is in blue (DAPI). (A) Nuclear NCC-1 expression levels increase during oocyte development (from right to left). A close-up of oocytes is shown in which DNA (B) and NCC-1 (C) are stained. NCC-1 localization is cytoplasmic during mitosis; (D) a wild-type 2 cell embryo in which the AB cell is in anaphase (left) and the P1 cell in metaphase (right). (E) Nuclear and cytoplasmic NCC-1 localization in a 4 cell wild-type embryo. (F,G) Comparison of NCC-1 staining in wild-type embryo (F) and
ncc-1(RNAi) embryo (G). Exposure times and processing were identical for both images. NCC-1 staining is absent in
ncc-1(RNAi) embryos. An arrow indicates condensed DNA in the paternal pronucleus. (H,I) NCC-1 (green) and DNA (PI, red) staining in wild-type and
ncc-1(RNAi) oocytes. NCC-1 accumulates in nuclei of developing oocytes (from right to left) in the wild-type (H) but not in dsRNA-injected gonads (I). Note that chromosomes fully condense (compare the earlier (right) and later (left) nuclei indicated) to the diakinesis state in oocytes following RNAi treatment. Scale bars approx. 10 µm.