Figure 1.
nrap-1 Encodes an LDLa-Domain Protein Expressed in Presynaptic Cells and Required for NMDAR-Dependent Behavior(A-D) Average forward duration (mean +- SEM). *p < 0.05, **p < 0.01, ***p < 0.001 by Mann-Whitney U test (A, C, and D) or Welch's t test (B). n = 8 for all genotypesin (A). For genotypes in (B), wild-type, n = 21;
ak365, n = 20;
gk874318, n = 20;
ak365; Pnrap-1::
nrap-1::mCherry, n = 13. n = 9 for all genotypes in (C). Forgenotypes in (D), wild-type, n = 11;
ky176, n = 12;
ok3474, n = 7;
ky176;
ok3474, n = 12.(E) NRAP-1 amino acid sequence. Signal peptide (boxed), conserved cysteines (red), residues mutated in
nrap-1(
gk874318) (asterisks) and
nrap-1(
ak365)(red arrowhead), region deleted in
nrap-1(
ok3474) (blue), and predicted N-linked glycosylation (black arrowhead).(F and G) Images of anterior (head) and posterior (tail) neuronal cell bodies in transgenic worms that co-expressed Pnrap-1::
nrap-1::SL2::gfp with eitherPeat-4::mCherry (F) or Pnmr-1::mCherry (G). GFP was tagged with a nuclear localization sequence. Scale bars, 10 mm.(H and I) Images of the VNC in transgenic worms that co-expressed Pnrap-1::gfp::
nrap-1 with either Peat-4::
eat-4::mCherry (H) or Pflp-18::mCherry::
nmr-2 (I).Scale bars, 5 um.(J) Images of a transgenic worm that expressed Pnrap-1::
nrap-1::mCherry. Arrowheads indicate coelomocytes. Scale bar, 5 um.See also Figures S1 and S2..