Figure 1.
mom-5/Frizzled and
cam-1/Ror2 Act in Parallel Genetic Pathways to Control the Anterior Migration of the QR Descendants(A) Schematic overview of the anterior migration of QR.a and QR.p and their descendants. Apoptotic cells are indicated as white cells with a cross, while the final QR descendants are indicated in green. The final position of QR.pa division is indicated in red.(B and E) Average position of the QR descendants QR.pap and QR.paa with respect to the seam cells V1.a to V6.p (lower brackets indicate Vn.a [left] and Vn.p [right] daughters of Vn cells). Values listed are percentiles of the total number of cells scored, n > 50 for all genotypes. A color (red) coded heatmap represents the range of percentile values. The
hsp16.2 HS promoter was used to drive ubiquitous expression of
egl-20 and
cwn-1, and the time of HS is indicated. The
ceh-22promoter was used to drive anterior expression of
egl-20 and
cwn-1 in the pharynx (Okkema and Fire, 1994). Q lineage specific RNAi was performed by expressing
cam-1 or
mom-5 dsRNA using the
egl-17 promoter (Burdine et al., 1998) in the RNAi spreading defective mutant
sid-1(
qt9) (Winston et al., 2002).Statistical significance was calculated using Fisher's exact test (***p < 0.0001).(C) Single-molecule mRNA FISH of
cam-1 and
mom-5 mRNA (red). The Q neuroblasts (outlined with dotted line) and seam cells are labeled with GFP (heIs63).Quantification of mRNA spots is indicated as mean ± SD (n > 30). Statistical significance was calculated using an unpaired t test (**p < 0.001).(D)
cam-1 and
mom-5 transcription dynamics in single QR.p (green) and QR.pa (red) neuroblast daughter cells as measured in wild-type animals (n > 60 for bothmRNA species). The number of mRNA spots per cell is plotted against the cell position with respect to the seam cells H2 to V5. See also Figure S1.