Figure 6. Cortical NMY-2 Flows and Maintenance of PAR Distributions after Pseudocleavage. (A-F) Cortical flows of NMY-2::GFP and PAR-6::GFP in wild-type and
par-2 (RNAi) embryos. (A, C, and E) Surface views of metaphase stage embryos. (B, D, and F) Kymographs from the same embryos. Arrow in (B) shows the anterior flow of NMY-2::GFP into a dense band at the rear edge of the anterior cap. In
par-2 (RNAi) embryos, aberrant posterior flows of NMY-2::GFP and PAR-6::GFP (arrows in [D] and [F]) return these proteins to the posterior cortex (C and E).(G-L) Embryos fixed at metaphase and costained for NMY-2 and PAR-2 (G-I) or NMY-2 and PAR-3 (J-L).