Figure 4. smo-1
::GFP is expressed in the entire somatic gonad. Nomarski photomicrographs (A,C,E) of wild-type animals expressing the smo-1
::GFP reporter and corresponding fluorescence images (B,D,F). (A,B) Ventral view of mid-L4 animal. Expression is detected in all vulval cells (vul), spermathecal cells (sp), hypodermis (hyp), seam cells (se), and ventral nerve cord (vnc). (C,D) Lateral view of mid-L4 animal. Expression is detected in all vulval cells (vul), spermathecal cells (sp), sheath cells (sh), all uterine cells (ut), and the distal tip cell (DTC). Inset shows transgene expression in the anchor cell of earlier-stage animals. (E,F). Lateral view of adult spermatheca. Expression is restricted to the somatic spermathecal cells (sp) and sheath cells (sh). There is no expression in sperm (sstarf;), oocytes (oo), fertilized egg (fe), or two-cell embryos (right of the fertilized egg). Expression in embryos was detected from the gastrulation stage (see Supplemental Material). Bar, 10 μm.