Fig. 7. Expression of
pad-2 in larval and adult stages. (A, B, C) Fluorescence micrographs showing PAD-2DGFP accumulation in the anterior, middle, and posterior body part of L2 larvae, respectively. (A) In the head, PAD-2DGFP is detectable in the nerve ring (arrow) and in the anterior hypodermal cells, hyp 3-6 (arrowheads). (B) In the midbody region, neurons that belong to the dorsal and ventral nerve cords indicated as dc and vc express
pad-2. (C) PAD-2DGFP also accumulates in all intestinal (i) and many hypodermal cells (hyp 11). (D) Expression of
pad-2 in a young adult hermaphrodite. Vulval cells show intense PAD-2DGFP accumulation. Arrows point to the spermatheca where
pad-2 is also active. (E) Enlarged nucleus of a pad-2Dgfp-expressing cell showing the presence of discrete foci of fluorescence that appear over the more diffuse background fluorescence. (F)
pad-2 is intensely expressed in the body wall muscle sarcomeres. (G) PAD-2DGFP accumulation in the posterior body part of an adult male shows
pad-2 expression in hypodermal, muscle, and neuronal cells. The bracket indicates the tail (rays). Bars represent 40 um.