Figure 8. spe-44
conservation in Caenorhabditis species. A. Tree indicating the relationship of the SPE-44 orthologs from the indicated species. CLUSTALW2  was used to perform multiple sequence alignment of complete amino acid sequences for the following proteins: Wormbase accession numbers CN01523 (C. brenneri; abbreviated Cbrn), RP47564 (C. remanei; Crem), CBP08202 (C. briggsae; Cbrg), CE05305 (C. elegans; Celg), and JA05534 (C. japonica; Cjap). Also included were the paralogous GMEB-3 from C. elegans (accession number CE31443; Celg GMEB-3), and human GMEB1 as an outgroup (NCBI accession NP_077808; Hs GMEB1). Branch length reflects the degree of similarity. B. Sex-biased expression. Relative read number from RNA-Seq data; female/hermaphrodite values were normalized to one for each species. Celg, C. elegans; Crem, C. remanei; Cbrn, C. brenneri, Cjap, C. japonica. C. Conservation of spe-44
upstream sequences. Blue indicates conserved sequence elements; asterisks indicate identical nucleotides. D. SPE-44 protein localization in C. remanei. Dissected male gonad co-stained with anti-SPE-44 antibody (green) and DAPI (red). Higher magnification (inset) reveals exclusion from a single DNA focus, presumably X (arrow).