Figure 2.
npr-1 and
glb-5 Polymorphisms Account for the Two Behavioral QTLs.(A) Genetic identification of QTLs on V and X. Turning responses of strains with transgenes bearing N2 or HW alleles of
npr-1, or N2 or HW alleles of
glb-5; transgenes were in bicistronic transcripts followed by SL2::GFP.
ad609 is a loss-of-function allele of
npr-1 in the N2 background; 'Het' indicates F1 progeny of a XQTL::HW and VQTL::HW; XQTL::HW cross. Complete datasets are in Figure S1.(B) Sequence polymorphisms in
glb-5 between N2 and HW. In N2, a 765 bp duplication covering the sixth exon truncates the predicted GLB-5 protein. cc, coiled-coil domain; ct, alternate C-terminal domain caused by the duplicated exon.(C) Expression of bicistronic Pglb-5::
glb-5::SL2::gfp transgene in L1 animal.(D) Rescue of XQTL:HW animals by expressing the HW allele of
glb-5 in URX, AQR, and PQR neurons (Pgcy-36::glb-5HW in bicistronic SL2::GFP clone; Pgcy-36::gfp::glb-5HW, N-terminally GFP-tagged glb-5HW).(E) Killing URX, AQR, and PQR with qaIs2241 eliminates the effects of glb-5HW.In panels (A), (D), and (E), control strains from Figure 1 are included for reference.