Figure S6. AP-3 at presynaptic region maintains the localization of axonal proteins. (Asso. w/ Figure 5)(A) GFP-tagged APM-3/AP-3μ is co-expressed with RFP-tagged SNB-1 in RIA. Scale bar is 10 μm.(B-D) RFP-tagged RAB-3 in RIA is mis-localized in
syd-2(
wy5) (B),
unc-104(
e1265) (C), and
ttx-7(
tm513)(D) mutant animals. Arrow points to the axonal region of RIA. Asterisk labels the dendrite of the RIA neuron. Scale bar is 10 μm. (E-F) GFP-tagged APM-3/AP-3μ (D) or SER-1 (E) is co-expressed with the cytosolic tdTomato insyd-2
(wy5) mutant animals. Scale bar is 10 μm. (G-H) GFP-tagged APM-3/AP-3µ (F) or SER-1 (G) is co-expressed with the cytosolic tdTomato in
unc-104(
e1265) mutant animals. Scale bar is 10 μm.(I-J) GFP-tagged APM-3/AP-3μ (H) or SER-1 (I) is co-expressed with the cytosolic tdTomato in
ttx-7(
tm513) mutant animals. Scale bar is 10 μm.