Supplementary Figure 2. Induction of
pgp-5 expression by infection and exposure to heavy metal. Fluorescence photomicrographs of
ppgp-5::gfp animals after 24 h continuous exposure to OP50 (A), PA14 (B), and OP50 with 100 μM CdCl2 (C) from the L4 larval stage. In every image, heads of the animals are on the left. Arrows point to the terminal bulb of the pharynx. Note that GFP expression in the terminal bulb is only detected in majority of animals treated with CdCl2. The scale bar represents 100 μm. (D) Quantification of GFP expression of
ppgp-5::gfp animals after 24 h continuous exposure to OP50 (blue; mean fluorescence = 21 ± 7), PA14 (red; mean fluorescence = 76 ± 29) or OP50 with CdCl2 100 μM (green; mean fluorescence = 43 ± 9) from the L4 larval stage using the COPAS biosort. Green fluorescence was significantly increased upon exposure to PA14 (Student's t-test, p < 0.0001) and exposure to cadmium (Student's t-test, p < 0.0001). Each spot represents one animal. The fluorescence and Time Of Flight (TOF) are measured in arbitrary, but constant units. TOF is a measure of the length of the animal; details can be found in [1]. ([1] C. Couillault, N. Pujol, J. Reboul, L. Sabatier, J.F. Guichou, Y. Kohara, and J.J. Ewbank, TLR-independent control of innate immunity in Caenorhabditis elegans by the TIR domain adaptor protein TIR-1, an ortholog of human SARM. Nat. Immunol. 5 (2004) 488-494.)