Figure 1. Neuron Positions and the Expression of MIG-10 Isoforms. (A) Summary of the positions of AVM and PVM relative to the sources of UNC-6 and SLT-1. For the circumferential migration, each axon is simultaneously attracted toward the UNC-6 sources (ventral epidermal cells and ventral nerve-cord neurons) and repelled away from SLT-1 sources (dorsal sublateral body-wall muscles).(B) Schematic of the
mig-10 gene structure and the transcriptional GFP fusion constructs for
mig-10a and
mig-10b. The
mig-10 gene includes two alternative translational start sites that produce two transcripts,
mig-10a and
mig-10b[9]. Four kilobases of sequence upstream of each translational start site was fused to GFP to produce the
mig-10a::gfp and
mig-10b::gfp transcriptional fusion constructs.(C) Photomicrographs of L4-stage animals showing
mig-10a::gfp transgene expression in the pharynx, in the vulva, and in a subset of neurons including the ALM, CAN, AVM, and PVM.(D) Photomicrographs of
mig-10b::gfp transgene expression in six head neurons (L2 stage shown) and in the intestine (L1 stage shown). Expression was not detected in AVM or PVM. Scale bars represent 20 um.