FIGURE 1. The kinesin KLP-4 functions in the VNC to regulate the abundance of GLR-1 and GLR-1-dependent behavior. (A) Protein domain organization of C. elegans KLP-4, Drosophila Khc-73 and mouse KIF13A and KIF13B. The N-terminal motor domain (red box) containing the signature ATP binding motif (thin black bar) and MT binding regions (thick black bar), the cargo binding domain (gray box), the Fork-head associated domain (FHA) (purple box) and the microtubule plus-end binding domain (CAP-Gly) (orange box) are shown. The percent identity of the motor domain of C. elegans KLP-4 (white percentages) or full length KLP-4 (black percentages) versus Drosophila Khc73, mouse KIF13A and KIF13B are indicated. (B-F) Representative images of the anterior VNC of larval stage 4 (L4) wild-type (B),
klp-4(
pz19) (C), rescued
klp-4(
pz19) (D),
klp-4(
tm2114) (E), and rescued
klp-4(
tm2114) (F) animals expressing an integrated GLR-1::GFP transgene (nuIs25). In these and all subsequent images anterior is to the left and ventral is up. (G-H) Quantification of GLR-1::GFP puncta intensities (normalized) (G) and densities (H) for the strains pictured in B-F. Means and SEM are shown for n = 38 wild type, n = 23
klp-4(
pz19), n = 21 rescued
klp-4(
pz19), n = 20
klp-4(
tm2114), and n = 21 rescued
klp-4(
tm2114) animals. (I) Quantification of number of spontaneous reversals per minute for n = 20 wild-type, n = 20
klp-4(
tm2114), n = 23 animals expressing an integrated
klp-4 transgene under the control of the
glr-1 promoter (
klp-4(xs)) (pzIs20), n = 14
glr-1(
n2461), and n = 13
glr-1(
n2461) animals overexpressing
klp-4 (
klp-4(xs)). Values that differ significantly from wild type are indicated by asterisks above each bar, whereas other comparisons are marked by brackets (**p 0.001, *p 0.01, #p 0.05, Tukey-Kramer Test).