Figure 4. UNC-24 Localizes with MEC-2 and Is Needed for Touch Sensitivity. (A) MEC-2 and UNC-24::GFP puncta colocalize in a PLM process.(B)
unc-24(
e138) enhances the touch insensitive phenotype of
mec-4(
u45)ts at 21C and
mec-6(
u247)ts at 15C. Animals were touched five times in the head (white bars) and five times in the tail (black bars) alternately and the total number of responses for each area was recorded (mean ± SEM, n = 29-30 for each).(C)
unc-24 animals have a somewhat temperature-dependent insensitivity to harsh touch that is not enhanced in double mutants with
mec-4(
u45) and
mec-6(
u247). Animals were treated as in (B) except that they were touched with a platinum wire probe instead of an eyebrow hair.