Figure 2. Analysis of
mdt-15 expression. (A) Relative levels of
mdt-15 mRNA (normalized to
ama-1 mRNA levels) in embryonic, larval, and adult N2 worms were determined by qRT-PCR. The bars represent averages of three independent experiments; error bars represent SEM. (B,C) Fluorescence and DIC micrographs of BC11928 worms at the early adult stage. The promoter of
mdt-15 drives expression in the intestine (B), and in head neurons (C). Bars: B, 129 μm; C, 49 μm. (Pha) Pharynx; (Neu) head neurons; (Vul) vulva; (Int) intestine.