(C) A Pnpr-12::NPR-12::GFP translational reporter strain was used to visualizeNPR-12 protein expression in PVD neurons. A 2.2 kb fragment upstream of thestart codon of npr-12
was used as the npr-12
promoter. White arrow: PVD cellbody; red arrow: PVD dendrite; yellow arrow: initial segment of PVD axon. Scalebar: 10 um.(D) A Pnpr-12::GFP strain was used to visualize the gene expression pattern ofnpr-12. A 2.2 kb fragment upstream of the start codon of npr-12
was used as thenpr-12 promoter. Representative images of mid-section, head region, and tailregion of an animal are shown in the top, middle and bottom panels, respectively.The white arrow in the top image points at the PVD cell body. The middle imageshows npr-12
expression in FLP neurons (white arrow points at FLP cell body), and the bottom image demonstrates npr-12
expression in PVC neurons andpossibly in PHA neurons as well. The neurons in yellow color are AIY neuronsfrom expression of co-injection marker Pttx-3::RFP. Scale bars: 50 um.