Figure 1. GFP::GLR2(Q)/(R) in C. elegans. A, Alignment of the M2 pore-forming domain from mouse GluR-A and GluR-B subunits and seven C. elegans glr genes, with the Q/R position marked by an asterisk. B, Graphic depiction of GFP::GLR-2, with the Q/R site and membrane domains M1-M4. C, Examples of transgenic worms expressing the
glr-2-derived transgenes. GFP::GLR-2 and integrated GFP::GLR-2(R) in the wild-type background are shown above. The middle and bottom panels show expression of soluble YFP driven by the same
glr-2 promoter used in the fusion constructs. In the middle panel, it is the only transgene expressed, whereas in the bottom panel, it is in a strain carrying an integrated
glr-1::CFP soluble protein fusion. The inset (bottom left) shows URY cell terminal processes, which express
glr-1 and not
glr-2, in contrast to the M1 pharyngeal neuron process that runs down the middle of the pharynx and only expresses
glr-2. Arrowheads point to the DVA neurons.