Figure 3.
nhr-67 negatively regulates
unc-5 in the L3 stage and positively regulates
zmp-1 in the L4 stage. (A)
unc-5::GFP expression in the LC during the L3 and L4 stages remains constant in wild-type animals (top row), but becomes progressively stronger in
nhr-67(RNAi) animals (bottom row). (B) Summary of the levels of
unc-5::GFP fluorescence in the LC of wild-type and
nhr-67(RNAi) animals. (C) Nomarski image of an animal overexpressing UNC-5 in the LC during the L3 and L4 stages. In this
nhr-67::
unc-5-expressing animal, the LC continues to migrate on the dorsal bodywall instead of turning ventrally in the mid-L3 stage. (D) The LC expresses
zmp-1::GFP during the L4 stage in wild-type animals. The gonad is outlined in yellow and the LC is indicated by an arrow. Nomarski, top; fluorescence, bottom. (E) In
nhr-67(RNAi) animals,
zmp-1::GFP is not expressed in the L4 stage LC. (F) Summary of the expression of fluorescent markers in the LC of wild-type versus
nhr-67(RNAi) animals in the L4 stage. Genes that are expressed throughout LC migration, such as
lag-2,
him-4,
mig-2 and
gon-1, are not regulated by
nhr-67. By contrast,
zmp-1 and
unc-5, which have specific L3 and L4 stage expression, are regulated by
nhr-67. Scale bars: 20 μm.