Figure 1 : A. Graph showing the fraction of males of the genotypes indicated that responded to wild-type hermaphrodites within 10 minutes. Error bars indicate 95% confidence intervals. At least 16 males of each genotype were assayed. For these assays, males were placed on plates containing 10 wild-type hermaphrodites. B. Graph showing the fraction of
egl-4 mutant males that responded when males were placed on plates containing 30 hermaphrodites. Error bars indicate 95% confidence intervals. C. Graph showing the proportion of turns initiated by males of the indicated genotypes that were successful. The criteria used to judge turns as good, sloppy or missed were those described previously (Loer and Kenyon, 1993): A turn was judged to be "good" if the male's tail was in continuous contact with the hermaphrodite throughout the turn. A "sloppy" turn is defined as a turn in which the male succeeded in making the turn but the tail lost contact with the hermaphrodite during the turn. A turn is said to have been missed if the male's tail sailed off the end of the hermaphrodite and contact was not reestablished. **** indicates P<0.0001 (Fisher' exact test). For turning assays, 30 hermaphrodites were placed on a thin bacterial lawn generated with 13 μl of a culture of OP50 bacteria. Under these conditions, 20/20
egl-4(
n477) and 27/28
egl-4(
n478) males responded to an hermaphrodite within 10 minutes and most responded within five minutes. D. Graph showing the percentage of males with ventrally curled tails at different time points after the addition of serotonin. Males were placed in M9 medium in the wells of microtiter plates and serotonin hydrochloride was added to give a final concentration of 20 mM. 10 males were placed in each well. A total of 60 animals was analyzed for each genotype. Error bars indicate 95% confidence intervals.