Figure 4. Loss of
scav-3 does not affect targetingof lysosomal hydrolases. Merged confocal fluorescent images of the hypodermis in wild-type (WT; A, C, E, G, I, and K) and
scav-3(
qx193) (B, D, F, H, J,and L) larvae coexpressing LAAT-1::GFP and GBA-3::mCherry (A and B), GBA-1::mCherry (C and D), CPL-1::mCherry (E and F), CPR-6::mCherry (G and H), Y105E8B.9::mCherry (I and J), or ASM-1::mCherry(K and L). The hydrolases were seen in LAAT-1::GFPpositive globular (arrowheads) and tubular (arrows) lysosomes in wild type and enlarged vesicular lysosomes (arrowheads) in
scav-3(
qx193) mutants. Bars, 10 um.