Figure 1. The
crt-1(
jh101) mutants are hypersensitive to TM stress. Percentages of progeny that were arrested at indicated stages of development or dead were plotted against the different concentrations of TM treated to (a) the wildtype,(b)
crt-1(
jh101) mutants, or (c)
csq-1(
jh109) mutants. TM and heat stress up-regulates the
crt-1 expression. GFP expression of the jhIs11 integration line under (d) normal condition, (e) TM (30ug/ml) stress, or (f) heat (30 C) stress. The scale bar represents 100 um. The magnification is 100 and the exposure time was 80 ms. (g) Mixed stage N2 worms were cultured on an NGM plate containing TM (30 ug/ml) for indicated times and the transcript level of
crt-1 or
hsp-3 were analyzed by real time PCR. Expression of
crt-1 and
hsp-3 was normalized with the
act-3, which encodes an invertebrate actin. The error bar represents standard deviation from three reactions. (h) Western blot analysis of the worms treated with or without TM stress (12 h). The CSQ-1 was used as a loading control.