Figure S4 Subcellular localization of yellow fluorescent protein (YFP)::MACO-1, YFP::MACO-1 TM, and YFP::MACO-1 CC. (A-C) Expression of YFP::MACO-1 in almost all neurons and cyan fluorescent protein (CFP)::TRAM (rER marker) in AFD neurons of
maco-1(
nj34) animals. This transgenic strain showed a partial-rescued phenotype (Figure S2C). (A) YFP::MACO-1. (B) CFP::TRAM. (C) Merged YFP::MACO-1 and CFP::TRAM images. YFP::MACO-1 was localized to peri-nuclear regions and co-localized with CFP::TRAM, suggesting that MACO-1 is localized to the rER. (D-F) Expression of YFP::MACO-1 TM and CFP::TRAM in AFD neuron of wild-type animals. (D) YFP::MACO-1 TM. (E) CFP::TRAM. (F) Merged YFP::MACO- 1 TM and CFP::TRAM images. YFP::MACO-1 TM was colocalized with CFP::TRAM, suggesting that MACO-1 TM is localized to the rER. (G-I) Expression of YFP::MACO-1 CC and CFP::TRAM in AFD neuron among wild-type animals. (G) YFP::MACO-1 CC. (H) CFP::TRAM. (I) Merged YFP::MACO-1 CC and CFP::TRAM images. YFP::MACO-1 CC was negligibly co-localized with CFP::TRAM, suggesting that MACO-1 CC is only slightly localized to the rER. (J, K) Expression of YFP::MACO-1 TM in almost all neurons in
maco-1(
nj34). This strain did not show the rescued phenotype (Figure S2C). (J) Merged DIC and YFP images. (K) YFP image. (L, M) Expression of YFP::MACO-1 CC in almost all neurons in
maco-1(
nj34). This strain showed little to no rescued phenotype (Figure S2C). (L) Merged DIC and YFP images. (M) YFP image. Dashed ellipse shows a nerve ring (i.e., ring-shaped zone where axons of many neurons overlap). YFP::MACO-1 CC is localized to not only cytoplasm but neurites. Adult animals were observed in all images. Scale bars: A-C and J-M= 5 um; D-I= 2 um.