Figure 5. Embryonic expression of UNC-130. (A) Cartoon of a wild-type embryo at approximately 260 min postfertilization (adapted from Sulston et al. 1983). Identified UNC-130-expressing cells are in red. Additional unidentified UNC-130-expressing cells visible in the plane of focus in B are not marked. LIN-26-expressing hypodermal cells seen at this developmental stage are in green (Labouesse et al. 1996). Cells in yellow coexpress UNC-130 and LIN-26. Hatched cells also coexpress, but are not visible in the plane of focus in B. ABp(l/r)aapapa cells are the precursors to the AWA and ASG neurons, and ABp(l/r)aapapp cells are the precursors to the ASI neurons (Fig. 3A). (B) Wild-type embryo at the same developmental stage as in A stained with UNC-130 antibodies (red) and LIN-26 antibodies (green). The AWA/ASG precursors are marked with arrows; ASI precursors are marked with an arrowhead. Cells marked with asterisks coexpress UNC-130 and LIN-26. Additional cells not visible in this plane of focus also express UNC-130 at this developmental stage. (C)
unc-130(
ev505) embryo stained with UNC-130 (red) and LIN-26 antibodies (green). No UNC-130 staining is visible, consistent with the null nature of this allele. Scale, 10 μm.