Figure 5.
lpd-3 Is Expressed in the Worm Intestine and in Mammalian Fat. (A)
lpd-3 is expressed in the intestine. Transgenic worms expressing an
lpd-3::GFP translational fusion were examined by bright-field and GFP fluorescence microscopy.(B) The human homolog of
lpd-3 is expressed in fat, brain, and testis. PCR with human
lpd-3 primers was performed with a panel of cDNA from 16 different human tissues as template. S9 ribosomal PCR is a loading control.(C) Mouse
lpd-3 is expressed at higher levels in induced 3T3-L1 (IND) than in uninduced (UN) 3T3-L1 cells. 3T3-L1 cells were grown with (IND) or without (UN) induction media and then harvested for RT-PCR. G3PDH is a loading control. PPAR-gamma is upregulated in induced cells, while Pref-1 is downregulated.(D) Mouse
lpd-3 is expressed at higher levels in the mouse embryonic day 14.5 fat enlagen than in adult fat. PPAR-gamma and adipsin, a marker of terminal differentiation, are expressed at higher levels in adult fat. The -RT samples had no reverse transcriptase included in the reaction.