Figure 5 Images of knock-in strains generated using SEC. mNG fluorescence was imaged in the indicated strains. Left: initial insertions, which are predicted to behave as transcriptional reporters. Right: marker-excised strains, which express mNG^3xFlag fused to the protein of interest. The follow strains are shown: mNG^SEC^3xFlag::
ebp-2, LP345; mNG^3xFlag::
ebp-2, LP346; mNG^SEC^3xFlag::
gex-3,LP361; mNG^3xFlag::
gex-3, LP362; mNG^SEC^3xFlag::
mex-5, LP366; mNG^3xFlag::
mex-5, LP367; mNG^SEC^3xFlag::
nmy-2, LP388; mNG^3xFlag::
nmy-2, LP389; mNG^SEC^3xFlag::
oma-2, LP390; mNG^3xFlag::
oma-2, LP391; mNG^SEC^3xFlag::
rap-1, LP394; mNG^3xFlag::
rap-1, LP395. Arrowheads indicate expected localization of the fusion proteins (see text for details). Scale bars, 10 um.