Fig 3.
unc-6/netrin signaling via the
unc-5/UNC5 receptor requires
lon-2/glypican. (A) The axon of PVM normally migrates ventrally in the wild type, but it can be forced to migrate dorsally by misexpressing the repulsive receptor
unc-5/UNC5. We quantified PVM since AVM could not be reliably identified (bothAVM and neighboring ALMR axons project dorsally in Pmec-7::
unc-5 transgenic animals.) (B) Upon misexpression of
unc-5/UNC5 in PVM, using the transgene Pmec-7::
unc-5, the axon of PVM projects dorsally in an
unc-6/netrin-,
unc-40/DCC-, and
unc-34/enabled-dependent manner. Loss of
lon-2/glypican partially suppresses this forced dorsal migration, indicating that
unc-6/netrin signaling depends on
lon-2/glypican. Scale bar, 5 um. Error bars are standard error of the proportion. Asterisks denote significant difference: *** p 0.001 (z-tests, p-values were corrected by multiplying by the number of comparisons). ns, not significant. Wild type (without evIs25) is the same as in Fig 1B.