Figure 6. BBS-5 Expressed in Ciliated Cells. (A) Schematic of the adult hermaphrodite. The boxed areas indicate the regions shown in (B) and (C).(B and C) Confocal images showing
bbs-5::gfp expression in the amphid and labial neurons and phasmids of the adult hermaphrodite. Anterior is toward the left in (A)-(K).(D-G) Confocal images of
bbs-5::gfp expression (D and E) and DiO uptake (F and G) in wild-type (D and F) and mutant (E and G) worms. Expression of
bbs-5::gfp is markedly reduced in
daf-19(
m86) mutant worms (compare D and E). Similar results were observed with four independent transgenic strains.(H-K) Images showing the GFP fluorescence patterns from strains carrying a translational (BBS-5::GFP) transgene; shown are the GFP staining patterns in the amphid/labial neuron (H) and phasmid neuron (I) basal bodies, along with DIC-merged images (J and K).(L-T) Confocal images of the ependymal cells of the lining of the mouse ventricles. (L, N, and O) Antibodies to gamma-tubulin are in green, preimmune serum is red, and DNA is blue. (M, N, and Q) Antibodies to gamma-tubulin are in green, antibodies to mouse BBS5 are in red, and DNA is in blue. The boxes in (L) and (M) indicate the regions enlarged in (N), (O), (P), and (Q). The box in (R) indicates the region enlarged in (S) and (T). Antibodies to BBS5 are in red, antibodies to acetylated alpha-tubulin are in green, and DNA is in blue (R, S, and T). BBS5 is found at the base of the cilia.