Figure 3. Suppression of daf-6
mutations requires loss of lit-1
in glia. (A) Image of an amphid sheath glial cell body expressing lit-1p
::NLS-RFP (red; in nucleus) and vap-1p
::GFP (green) (transgene nsEx2308). Yellow, overlapping expression. Left is anterior. Scale bar, 10 mm. (B) Image of an adult (head) expressing lit-1p
::GFP (green) and ptr-10p
::NLS-RFP (red) (transgene nsEx2159). Arrows, cells with overlapping expression. Left is anterior. Scale bar, 10 mm. (C) Dye-filling assay for indicated genotypes (n$90). None of the transgenes had an effect on the dye filling of wild type animals (n.100, unpublished data). Error bars, SEM. p value calculated using Chi-squared test.