Figure 4. Expression of Wnt Pathway Components Controlling P7.p Orientation. Corresponding Nomarski (bottom) and epifluorescence (top) images are shown. The anchor cell is indicated by large arrowheads. Identities of VPC descendants are indicated below. Scale bar = 20 μm.(A) Full-length (C-term) LIN-18::GFP fusion under the control of the
lin-18 promoter. Expression in P5.p and P7.p granddaughters is shown. Although some puncta of GFP fluorescence were observed with this and other fusions, all TM domain-containing GFP fusions were on the plasma membrane.(B) Nuclear localized GFP under the control of the
lin-17 promoter in P5.p and P7.p granddaughters. Arrows point to ventral cord neurons that also express
lin-17::gfp. Although animals photographed in (A) and (B) display brighter expression in P5.p and P7.p compared to P6.p, many individuals express
lin-17::gfp and
lin-18::gfp in P6.p granddaughters also.(C) Full-length LIN-18::DsRed2 fusion protein under the control of the
lin-18 promoter (shown in red and membrane localized) and nuclear localized GFP under the control of the
lin-17 promoter. Expression in P7.p daughters is shown. P5.p and P6.p daughters also express
lin-17::gfp and
lin-18::gfp (or DsRed2) (not shown).(D) Membrane localized
lin-18::gfp in VPCs, P6.p and P7.p.(E)
lin-17::gfp in VPCs, P6.p, and P7.p. P5.p also expresses
lin-17::gfp and
lin-18::gfp (not shown). Downward arrows point to ventral cord neurons expressing
lin-17::gfp.(F) GFP under the control of the
mom-2 promoter in the anchor cell after the first division of VPCs.(G) GFP under the control of the
mom-2 promoter in the anchor cell and VPC granddaughters, P5.ppa, P5.ppp, P7.paa, and P7.pap (red arrows).