Figure 4. Expression and function of
asd-2b in the body wall muscles. (A) Schematic representation of the
asd-2 transcriptional fusion minigenes
asd-2a::GFP and
asd-2b::RFP. Green and magenta lines indicate the
asd-2a promoter and the
asd-2b promoter regions, respectively, used in the constructs. (B) Confocal images of the expression patterns of the
asd-2 transcriptional fusions in an adult (left column) and a late embryo (right column). Expression of
asd-2a::GFP (green) is detected in hypodermis (hyp) and pharyngeal muscles (phx). Expression of
asd-2b::RFP (magenta) is detected in the body wall muscles (bwm) and the pharyngeal muscles (phx). (Bottom) A DIC image of the same field. Bars: left column, 100 μm; right column, 50 μm. (C) Microphotograph of the
let-2 reporter allele ybIs1371 in the
asd-2 (
yb1423) mutant background carrying an extrachromosomal array for ASD-2b expression under the
myo-3 promoter. Arrowheads indicate young larvae and an embryo (shown in inset) carrying the array. Arrows indicate the pharynges of transgenic worms expressing GFP from
myo-2::GFP, a coinjected marker of transformation. Bars: 100 μm; inset, 50 μm. (D) Summary of the effects of
asd-2 expression on the
let-2 reporter.