Figure 2. Expression Pattern of
lat-1 and
lat-2. (A)
lat-1 mRNA in early blastomeres and (B) the gonad detected by in situ hybridization. (C and D) Zygotic expression of
lat-1 in early embryonic development.(C) Expression of a
lat-1p::gfp reporter gene at 26-cell stage. AB descendants are labeled 'alaa' for ABalaa, etc.(D) Membrane localization of a rescuing in-frame LAT-1::GFP fusion protein, 200-cell stage.(E and F) Expression of
lat-1 and
lat-2 overlaps in the pharynx primordium. (E)
lat-1p::gfp expression with strong signal in the pharyngeal primordium ('P') and ventral epidermal precursors ('E'). (F)
lat-2p::gfp expression at the same stage is confined to the pharynx primordium.(G and H) Asymmetric expression of
lat-1 in epidermal precursors during dorsal intercalation visualized with a
lat-1p::gfp transgene (G) or by detection with an anti-LAT-1 antibody. Arrows indicate position left seam cells, and arrowheads mark LAT-1-positive intercalating left dorsal epidermal cells. Scale bars in (C), (G), and (H) = 10 um, in (D), (E), and (F) = 5 um.