FIG. 3. Localization of
cct-1 transgene expression. (a)
cct-1-promoter-lacZ construct, which contains 2.7 kb of upstream noncoding region, the first intron in the
cct-1 gene, followed by 32 amino acids of the
cct-1 coding region fused in-frame to the nuclear localization signal (NLS) and b-galactosidase gene, as well as approximately 1000 bp of
cct-1 3'-noncoding region (including the polyadenylation signal). SL1, trans-splicing signal. (b-f) Expression pattern of selected nematodes (see text for particulars). T, tail; NC, ventral nerve cord; PH, pharynx; CB, copulatory bursa of male nematode; HYP, hypodermal cell.