FIGURE 1: Promoter analysis of
unc-87A and
unc-87B. (A-N) Promoter activity of
unc-87A (A-H) and
unc-87B (I-N) was examined using GFP as a reporter. Each fluorescence micrograph of GFP (black and white) is paired with a differential interference contrast image overlaid with green fluorescence of GFP. Images of whole worms with their heads on the left (A, B, I, and J) and magnified views of representative tissues (C-H, K-N) are shown. Note that the micrograph in C is somewhat overexposed for GFP in the pharynx to demonstrate relatively weak GFP in the neurons. (O-W) Expression ofGFP in hermaphrodite gonads with no transgene (O-Q), Punc-87A::GFP (R-T), or Punc-87B::GFP (U-W) was examined by immunostaining of dissected gonads for GFP (O, R, and U) and MYO-3 as a marker of myoepithelial sheath (P, S, andV). Merged images are shown in Q, T, and W (green, GFP; red, MYO-3; blue, DNA [not shown as individual images]). Scale bars, 100 um (whole worms; A, B, I, and J), 20 um (magnified tissues; C-H,K-N), 50 um (dissected gonads; O-W).