Figure 1. Hypoxia evokes food leaving response in C elegans:(A) Representative images showing food leaving of wild type animals (N2) in 1% O2 for 0, 12 and 24 hours. (B) Lawn occupancy of wild type animals in the indicated O2 concentrations for 0, 4, 12 and 24 hours. Error bars indicate standard error of the mean (SEM). Three independent experiments were performed with three technical replicates for each strain in each experiment. *, p<0.05; ***, p<0.001; ****, p<0.0001; NS = not significant. One way ANOVA with Tukey's test. (C) Video tracking of lawn occupancy of wild type animals in 1% O2 for 4 hours. 5 videos were acquired. The average lawn occupancy of 5 videos was plotted every 10 minutes. Error bars indicate SEM. (D) Lawn occupancy of wild type animals on dead bacteria for 0, 4, 12 and 24 hours in 21% O2 (blue) and 1% O2 (red). Three independent experiments were performed with three technical replicates for each strain in each experiment. **, p<0.01; ****, p<0.0001. One way ANOVA with Tukey's test. (E) Lawn occupancy of wild type,
dyf-3(
m185),
bbs-9(
gk471) and
gpa-3(
pk35)
odr-3(
n1605) in 21% O2 (blue) and 1% O2 (red) for 24 hours. Three independent experiments were performed with three technical replicates for each strain in each experiment. **, p<0.01; NS = not significant. One way ANOVA with Tukey's test. (F) Lawn occupancy of wild type,
gas-1(
fc21) and
isp-1(
qm150) mutants in 21% O2 (blue) or 1% O2 (red) for 24 hours. Three independent experiments were performed with three technical replicates for each strain in each experiment. ***, p<0.001; NS = not significant. One way ANOVA with Tukey's test. (G) Lawn occupancy of wild type,
hif-1(
ia4) and
egl-9(
sa307) in 21% O2 (blue) and 1% O2 (red) for 24 hours. Three independent experiments were performed with three technical replicates for each strain in each experiment. NS = not significant. One way ANOVA with Tukey's test. (H) Lawn occupancy of wild type
gcy-35(
ok769),
tax-4(
p678) and qaIs2241 in 21% O2 (blue) or 1% O2 (red) for 24 hours. In the qaIs2241 strain, the O2 sensing neuron URX, AQR and PQR were genetically ablated. Three independent experiments were performed with three technical replicates for each strain in each experiment. NS = not significant. One way ANOVA with Tukey's test. (I) Lawn occupancy of wild type,
egl-3(
ok979) and
egl-21(
n476) in 21% O2 (blue) or 1% O2 (red) for 24 hours. Three independent experiments were performed with three technical replicates for each strain in each experiment. ****, p<0.0001. One way ANOVA with Tukey's test.