Figure 1. Mutations in the DGET motif of TAT-5 alter fertility, phagocytosis, and extracellular vesicle (EV) release:(A) Embryos and oocytes were laid by
tat-5(D244T) mutant hermaphrodites (n=11), but rarely by
tat-5(E246Q) mutant hermaphrodites (n=10). (B-C) The phagocytosis of P0 midbody remnants labeled with NMY-2::mCh (B) or second polar bodies labeled with mCh::PH::CTPD (C) was significantly disrupted in
tat-5(D244T) mutant embryos (***p<0.001 Fisher's exact test). (D) The number of mCh::PH::CTPD puncta on the surface of individual
tat-5(D244T) mutant embryos (n=18) was significantly increased compared to control embryos (n=20, ***p<0.001 Student's t-test). (E-F) Surface of 4-cell embryos expressing mCh::PH::CTPD in control (E) and
tat-5(D244T) mutants (F).