(I-L) The localization of BLOS-9::GFP (I) andmCherry::RAB-3 (J) in the dorsal synaptic region.The merged image is shown in (K). (L) is a zoomedin image of the boxed area in (K). BLOS-9 andRAB-3 are well co-localized. Scale bars, 50 um.See also Figure S5.
In situ hybridization images on embryonic, larval, and adult worms with vha-6 (I-L) are shown. vha-6 is expressed only in the intestine, from the embryonic coma stage (I) through larvae (J and K) and adult (L).
Immunofluorescence analysis of PGL-1 protein distributions in embryos. Anterior is left; ventral is bottom. Wild-type early embryos were costained with affinity-purified rabbit anti-PGL-1 antibody (G, I, and K). PGL-1 is associated with P granules in the germline blastomeres. Shown are P0 (A), P2 (I), and P4 (K) of 1-, 4-, and 44-cell embryos, respectively.
2. Hashimshony, T., Feder, M., Levin, M., Hall, B. K. & Yanai, I. Spatiotemporal transcriptomics reveals the evolutionary history of the endoderm germ layer. Nature 519, 219-22 (2014).
2. Hashimshony, T., Feder, M., Levin, M., Hall, B. K. & Yanai, I. Spatiotemporal transcriptomics reveals the evolutionary history of the endoderm germ layer. Nature 519, 219-22 (2014).
2. Hashimshony, T., Feder, M., Levin, M., Hall, B. K. & Yanai, I. Spatiotemporal transcriptomics reveals the evolutionary history of the endoderm germ layer. Nature 519, 219-22 (2014).
2. Hashimshony, T., Feder, M., Levin, M., Hall, B. K. & Yanai, I. Spatiotemporal transcriptomics reveals the evolutionary history of the endoderm germ layer. Nature 519, 219-22 (2014).
2. Hashimshony, T., Feder, M., Levin, M., Hall, B. K. & Yanai, I. Spatiotemporal transcriptomics reveals the evolutionary history of the endoderm germ layer. Nature 519, 219-22 (2014).
2. Hashimshony, T., Feder, M., Levin, M., Hall, B. K. & Yanai, I. Spatiotemporal transcriptomics reveals the evolutionary history of the endoderm germ layer. Nature 519, 219-22 (2014).