Figure 2. Transgenic rescue and cloning of
islo-1. (A) The first curvature angle obtained from wild-type (N2) and
islo-1(
eg978), and
islo-1 transgenic rescue strains (genomic rescue strain,
islo-1;Ex[
islo-1], and muscle specific rescue strain,
islo-1;Ex[P
myo-3 islo-154-end]). Error bars, SEM. Asterisks represent significant difference between
islo-1 and other animal groups (P<0.05). (B) Representative photos show the anterior portion of animals. (C) The predicted amino acid sequence of ISLO-1. The predicted transmembrane domain sequences are underlined and the type I PDZ recognition sequence is bold. (D-G) The expression pattern of the
islo-1 gene. The upstream sequence of the predicted translation initiation site was inserted into a GFP coding sequence in frame, and the resulting DNA was used for generating transgenic animals in wild-type animals. Expression was observed in neurons (D), body-wall muscles (E), egg-laying muscles (F) and enteric muscles (G). Scale bar, 10 um.