Figure 4
sacy-1 mutations suppress
acy-4(lf) sterility. (A)
sacy-1 alleles isolated as
acy-4(lf) suppressor mutations are shown. The
sacy-1(
tm5503) deletion is underlined. (B) C. elegans SACY-1 is highly conserved. ClustalW alignment of SACY-1, Drosophila Abstrakt (Irion and Leptin 1999; Schmucker et al. 2000), and human DDX41. SACY-1 and Abstrakt share 54% (323/603) identity and 70% (424/603) similarity; SACY-1 and DDX41 share 60% (318/533) identity and 75% (401/533) similarity.
sacy-1 mutant alleles (triangles) and the DEAD box (boxed in red) are indicated. Ce_SACY-1 (NP_491962.1), Dm_Abstrakt (NP_524220.1), and Hs_DDX41 (NP_057306.2) were used for the analysis. Conserved domains [DEAD-box domain (DEADc), helicase domain (HELICc), and zinc finger domain (ZnF)] and motifs (Q, I, Ia, Ib, II, III, IV, V, and VI) are indicated (Henn et al. 2012). (C) Rescuing GFP::SACY-1 fusion (tnEx159) is broadly expressed in the nuclei and cytoplasm of most or all cells. Embryos (e), spermatheca (sp), oocytes (-1, -2, and -3). Bar, 50 um.