Figure 5. Expression of Cullin mRNAs. (A) Northern blot of total RNA from wild-type embryos (E), larvae (L1, L2, L3, L4), and pregravid adults (A). The same membrane was probed sequentially with
cul-1,
cul-2,
cul-3,
cul-4, and 18S rRNA cDNAs.(B) Northern blot of poly(A)-selected RNA from mature wild-type,
fem-1(
hc17), and
glp-4(
bn2) adults cultured at 25 C. The membrane was probed sequentially with
cul-1 and, for comparison, with
cey-2 and
act-1 cDNAs. In wild type,
cey-2 mRNA is found only in germline cells and developing embryos (W. Kelly, personal communication).