Figure 5. Tissue-specific expressions of the
tmy-1 gene. A fusion gene containing the
tmy-1 gene and lacZ was used to study cellular expression. C. elegans preparations were histochemically stained for b-galactosidase expression using X- Gal as a substrate (A to F) and for tropomyosin isoforms with affinity-purified antibodies (G & H). A, Staining of the body wall muscles, pTMIZ2328, L3; B, staining of the male tail muscles, pTMIZ2328; C, staining of body-wall muscles, pT- MIZ2328, adult; D, staining of the pharyngeal muscles, pTMIII3256, adult; E, staining of L1 worm, pTMIZ818; F, staining of the pharyngeal muscles, pTMIIIZ3256-Bg, L1 worm; G, staining of the body-wall muscles with affinity-purified anti- CeTMI; H, staining of the pharyngeal muscles with affinity-purified anti-CeTMIII. Scale bars represent 50 um. These results indicate that the 5' end promoter and the internal promoter in the third intron control the expression of the body wall and the pharyngeal muscles, respectively.