Figure 1. CeTLF Is Expressed Ubiquitously in Early Embryos. (A) Northern blots of early embryonic mRNA were probed for
tlf-1 (left) or Cetbp (right; Lichtsteiner and Tjian 1993). The minor species below the
tlf-1 band is not seen reproducibly (M. Horner and S. E. M., unpublished data). The open arrow denotes unspliced RNA, and the closed arrows denote
tlf-1 (approximately 2.8 kb) and Cetbp (approximately 1.7 kb) mRNAs. 'M' refers to size markers.(B) Mouse embryonic Northern blot probed sequentially with a 1.5 kb fragment from mouse TLF (left) or TBP (right) cDNA. TLF produces two mRNAs of approximately 3.5 kb and 1.9 kb, indicated by arrows. TBP produces an mRNA of approximately 2.4 kb. 'dpc' denotes days post coitum. See Dantonel et al. 1999 for a sequence comparison of mouse and worm TLF.(C) Immunofluorescence for CeTLF (left) or CeTBP (right) in 2-cell (top) or 3-fold (middle) wild-type embryos. Bottom panels show CeTLF (left) and P granule (right) staining in a
tlf-1(RNAi) embryo at approximately the 28-cell stage. Each embryo is 50 um.