Figure 2.
kvs-1 and
mps-1are broadly expressed in C. elegans nervous system. A, fluorescence microscopy image taken from a P
mps-1::gfp transgenic nematode head. Anterior is to the left. This worm expresses GFP in ADF neurons. B, DiD staining of amphid neurons of the same nematode in A. Transgenic animals were incubated with a fluorescent dye (DiD) that stains specifically some amphid neurons. The image was taken with rhodamine filter sets on the same focal plane as in A. C, merged images (A and B) demonstrate overlap of green and red signals in the ADF neuron. D-F,P
mps-1::gfp fluorescence in ASH neurons. Same as in A-C on a different focal plane. DiD does not constitutively stain on ASE and ASG neurons. G, fluorescent microscopy image showing
mps-1 expression of GFP in an ALM touch-neuron. H,
mps-1 expression in tail PLML touch-neuron (PLMR is on another focal plane, not shown).I-K,
kvs-1 head expression pattern. Anterior is to the left. This worm expresses GFP in head neurons, including ADLs and ASKs. L-N, same as in panels I-K on a different focal plane showing
kvs-1expression in ASH neurons. O-Q,
kvs-1 expression in AWC, ASE, and ADF neurons. R,
kvs-1 expression in ventral cord neurons VAs, VBs, and DBs. Not all VA, VB, and DB neurons are visible on this focal plane. S, fluorescence image showing expression of
kvs-1 in anal depressor muscle.