Figure 6. PKD-2::GFP CEM ciliary distribution in wild type and IFT mutants. The first column depicts a cartoon showing two out of four CEM cilia in the male nose. Column 2 shows PKD-2::GFP ciliary distribution patterns. Column 4 shows Ppkd-2::TBB-4::DsRed2 labeling CEM ciliary axonemes of wild type and IFT mutants. Column 3 is the merged image of Columns 2 and 4. Scare bars: 5 μm. (A) In wild type, PKD-2::GFP is enriched in the ciliary base (transition zone and distal-most dendrite, bracket) and along the ciliary membrane (dashed bracket). (B-E) PKD-2::GFP accumulates in the ciliary bases (bracket) and abnormal cilia (dashed bracket) of IFT mutants (B,C) In
osm-3 and
osm-5 mutants, PKD-2::GFP accumulates at the bases of stunted cilia. (D) In the
che-3 cytoplasmic dynein mutant, cilia form and PKD-2 accumulates at the ciliary bases and along the ciliary membrane. (E) In the
klp-11 kinesin II mutant (and
kap-1, not shown), CEM cilia morphology is abnormal and PKD-2 accumulates at ciliary bases and along the ciliary membrane. (F) Kinesin II is expressed in polycystin-expressing neurons. The polycystin-expressing neurons are labeled with a
pkd-2 promoter driven DsRed2 (left panel). KAP-1::GFP (right panel) is enriched in the ciliary compartment, but also found in dendrites (arrow) and axons (arrowhead). HOB, hook B neuron. (A) Reproduced, with permission, from Qin et al. (Qin et al., 2005).