Figure 1. Localization of CYE-1, CDC-25.1, and PLK-1 in wild-type embryos. (A and B) CYE-1 (A) and CDC-25.1 (B) immunostaining (green) is shown on top; costaining of DNA (DAPI, blue) and tubulin (red) is shown on the bottom. CYE-1 is found at equal levels in AB and P1 nuclei; AB nuclei show higher levels of CDC-25.1 than P1 nuclei from the early two-cell stage onward. (C) The
plk-1(RNAi) embryo has reduced nuclear level of CDC-25.1 compared with the wild type but retains asymmetry of distribution (right, high contrast [HC] image; dotted line marks nuclear envelope). (D) PLK-1 is shown on top (green); DNA (DAPI, blue) and tubulin (red) are shown on the bottom. Anterior cytoplasmic enrichment of PLK-1 is observed from pronuclear migration onwards. Bar, 10 um.